![]() ![]() In most basic terms, it is used to detect the presence of a specific protein in a complex mixture extracted from cells or tissue. Western blotting, also known as immunoblotting or protein blotting, is a core technique in cell and molecular biology. Illuminating the Pathway to Confident Western Blot Detection of Phosphorylated Proteins Western Blot Detection of Phosphorylation Events ![]() Positive control lysates - provide researchers confidence in their data by simplifying validation of antibody specificity and troubleshooting of western blotting protocols.įor more information, please visit. Trial sizes for all antibodies - enable researchers to test the antibody and optimise experimental conditions without a large upfront investment.ĭetailed validation protocols - streamline optimisation and validation, saving time and reagents and delivering top results These antibodies also offer numerous benefits for optimising protocols and validating antibody specificity in a researcher’s sample, which include: Bio-Rad shows the entire western blot image used for validation, giving the customer a complete view of antibody quality. 'Reliability is always a concern with antibodies, but Bio-Rad’s approach to quality control gives me considerable confidence that its products will stand apart from current offerings.'Īccording to Bio-Rad, the beta testers also valued the greater depth of information these antibodies provide. 'From what we have seen testing an initial range of PrecisionAb antibodies, the product performs well with little optimisation and at low concentration,' said Subhash Verma, PhD, an associate professor of molecular microbiology and immunology at the University of Nevada, Reno. A, this carbonic anhydrase IX (CA9) mouse monoclonal antibody failed validation due to nonspecific binding and low signal-to-noise ratio B, this thymidylate synthase (TYMS) mouse monoclonal antibody passed validation showing high specificity and sensitivity. Each new lot is screened before release to ensure lot-to-lot reproducibility.Ī worldwide trial, performed in a range of laboratory environments, confirmed the high specificity and sensitivity of the antibodies, says Bio-Rad. Only antibodies that detect endogenous protein levels with high sensitivity and specificity are included in the PrecisionAb product line. Customers can have confidence in their antibodies and their results.'īio-Rad’s PrecisionAb antibodies are screened using whole cell lysates from up to 12 different biologically relevant cell lines - by far the industry’s most rigorous validation process, Bio-Rad says. Our stringent validation and quality control ensures only top-performing antibodies are shipped along with extensive validation data. 'This discrepancy has created much distrust in the market. 'There’s a huge discrepancy between the claims of antibody performance from vendors and what scientists encounter when the actual product arrives,' said Tiffani Manolis, marketing manager for protein quantitation at Bio-Rad. ![]() ![]() Scientists have struggled with antibody unreliability for decades because manufacturing standards and quality control vary widely among vendors, targets, and even among antibody lots. These are the first of thousands of PrecisionsAb antibodies Bio-Rad plans to release to help researchers improve western blotting results. They also come with very complete validation data so that researchers can accurately assess the antibodies’ performance before buying, and a positive control lysate so that researchers can optimise the antibodies in their own labs. These antibodies are designed to offer superior sensitivity, specificity and reliability. Detailed validation protocols streamline optimisation and validation, saving time and reagentsīio-Rad Laboratories has released the first set in a new product line of PrecisonAb antibodies, rigorously validated for use in western blotting. ![]()
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